ExpressPlex™ HT NGS library preparation kit containing 6,144 indices can significantly reduce bottlenecks in plasmid and amplicon sequencing for synthetic biology, gene editing, and protein engineering applications

seqWell, a global provider of genomic library workflow solutions, today announced the launch of their new ExpressPlex HT Library Preparation Kit (ExpressPlex HT), the first commercially available next-generation sequencing (NGS) library preparation kit containing all the required reagents and indices to enable multiplexing up to 6,144 samples in a pre-plated in 384-well format. ExpressPlex HT uses a 90-minute workflow in a single mastermix reaction to streamline NGS library preparation through massive multiplexing, thereby enabling lower cost, high throughput sequencing of plasmids, amplicons and other synthetic constructs used in synthetic biology.

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seqWell's ExpressPlex HT 384-well library prep kit is the first commercially available next-generation sequencing library preparation kit enabling multiplexing up to 6,144 samples in a pre-plated 384-well format. (Graphic: Business Wire)

“ExpressPlex HT was a natural step in broadening our portfolio of scalable, multiplex library preparation solutions,” said Jack Leonard, Chief Technology Officer at seqWell. “We took a holistic view of our customers’ workflows to address challenges across their ultra-high throughput plasmid sequencing processes, including reagent dispensing and inventory management. Sample-specific barcoding and library amplification both take place in a single reaction step. ExpressPlex HT has brought significant improvements in workflow efficiency to our early access customers.”

Key features of ExpressPlex HT that bring a new level of scale and productivity to plasmid and amplicon NGS:

• Only commercially available, ready-to-use 384-well library preparation solution. Reagents and indices are supplied in a pre-plated, 384-well format, eliminating the need for reagent dispensing and the complex supply chain management of barcodes and primers.
• Streamlined, ultra-high throughput and automated workflow. Massive multiplexing using 6,144 indices and a fully automated, 90-minute library preparation workflow enable 24-hour sample-to-answer turnaround for greater than 6,000 samples.
• Lower environmental impact. ExpressPlex HT’s pre-plated reagents, workflow simplicity, and massive multiplexing require less than 10% of the tips and plastics required for other commonly used high throughput library prep kits, providing large savings in consumable budget and up to a 90% reduction in consumables waste.

“High-throughput synthetic biology increasingly relies on repeated cycles of DBTL – design, build, test and learn – where sequencing often plays a crucial role,” said Joe Mellor, Co-Founder and Chief Scientific Officer at seqWell. “Today’s synthetic biology experiments frequently involve thousands of nucleic acid-based synthetic samples such as plasmids and amplicons, and the difficulty of streamlining NGS library preparation and sequencing for these samples is a bottleneck. ExpressPlex HT can alleviate that bottleneck and more efficiently develop actionable insights from ultra-high throughput experiments and production-scale sequencing of synthetic constructs.”

seqWell recently presented data for ExpressPlex HT library preparation kits in a poster entitled, “Ultra-High Throughput Sequencing for Synthetic Biology Discovery,” at the Advances in Genome Biology and Technology (AGBT) meeting. To review the data, visit the ExpressPlexHT_AGBT poster.

About seqWell

seqWell creates scalable genomics technologies that simplify workflows and help enable every life scientist to unlock transformative discoveries with sequencing. seqWell offers a range of next-generation library prep products and workflows that allow customers to achieve higher levels of sample multiplexing quickly and easily. Learn more about us at www.seqwell.com or follow us on Linkedin.

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